| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 821 | Chlorobium limicola | ACGACTGGACGGCATTCA | TTCGGTCTGTGCGCTGTT | 58.94 | 59.89 | 238 | 55 |
100.00%
|
100.00%
|
| Primer 822 | Chlorobium limicola | ATTGCCGAAGCTCTGCCT | ACTGCAGCTTGAGGGCAA | 59.33 | 59.48 | 211 | 55 |
100.00%
|
100.00%
|
| Primer 823 | Chlorobium limicola | ATGGACCGCTCGCTTATCG | ATGGCCAGCGACATGGTT | 60.01 | 59.64 | 203 | 55 |
100.00%
|
100.00%
|
| Primer 824 | Chlorobium limicola | ACAAGCTGCAGCACGGTT | AACGCTTCGATGAGAGCGG | 60.52 | 60.52 | 157 | 55 |
100.00%
|
100.00%
|
| Primer 825 | Chlorobium limicola | TCGTCACCGGAGCATTGT | AGGCCATCTGCTGCTGTT | 58.94 | 59.24 | 267 | 55 |
100.00%
|
100.00%
|
| Primer 826 | Chlorobium limicola | AAGCCTTTGCCAGCCGTT | ATGGCTCCAAGGCTTGCA | 60.52 | 59.56 | 211 | 55 |
100.00%
|
100.00%
|
| Primer 827 | Chlorobium limicola | GCACCGGTACAGTTCGTGA | TTGCAACCGAGCTGCCTA | 60.01 | 59.25 | 226 | 55 |
100.00%
|
100.00%
|
| Primer 828 | Chlorobium limicola | TCGCGTTTGTCTGCACCT | AGTTGCCGTGGGTGAAGT | 59.89 | 59.08 | 210 | 55 |
100.00%
|
100.00%
|
| Primer 829 | Chlorobium limicola | TCGCGTTTGTCTGCACCT | TCCGCACCTTTCTGCAGAG | 59.89 | 60.00 | 154 | 55 |
100.00%
|
100.00%
|
| Primer 830 | Chlorobium limicola | TCGCGTTTGTCTGCACCT | GGTAGTTGCCGTGGGTGAA | 59.89 | 59.93 | 213 | 55 |
100.00%
|
100.00%
|